Reporter

Part:BBa_K187019:Design

Designed by: Team Biobytes   Group: iGEM09_Alberta   (2009-10-17)


RFP in pAB, Biobytes plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
    Illegal PstI site found at 697
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 697
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
    Illegal PstI site found at 697
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
    Illegal PstI site found at 697
    Illegal AgeI site found at 570
    Illegal AgeI site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This plasmid includes only the open reading frame (start codon to stop codon) of RFP. A promoter and terminator are not included. pAB and pBA do include an RBS consensus sequence positioned 8 bp upstream of the ATG. We recommend that to express RFP, you use the Biobytes assembly method together with the promoter and terminator parts we have submited in pAB and pBA to assemble promoters and terminators onto RFP. As there is a range of promoters to chose from, this allows rapid manipulation of gene expression level. Using the Biobytes method, several DNA segments can be combined in just 20min per segment.


Source

Part BBa_J23018 was the source of RFP. Biobytes plasmid pAB is entered as BBa_K187000.

References